Identification of a Chitin-Binding Protein Secreted by Pseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
Identification of a chitin-binding protein secreted by Pseudomonas aeruginosa.
One of the major proteins secreted by Pseudomonas aeruginosa is a 43-kDa protein, which is cleaved by elastase into smaller fragments, including a 30-kDa and a 23-kDa fragment. The N-terminal 23-kDa fragment was previously suggested as corresponding to a staphylolytic protease and was designated LasD (S. Park and D. R. Galloway, Mol. Microbiol. 16:263-270, 1995). However, the sequence of the ge...
متن کاملCharacterization of Pseudomonas aeruginosa chitinase, a gradually secreted protein.
The gram-negative bacterium Pseudomonas aeruginosa secretes many proteins into its extracellular environment via the type I, II, and III secretion systems. In this study, a gene, chiC, coding for an extracellular chitinolytic enzyme, was identified. The chiC gene encodes a polypeptide of 483 amino acid residues, without a typical N-terminal signal sequence. Nevertheless, an N-terminal segment o...
متن کاملPslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.
The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...
متن کاملThe effector protein ExoY secreted by Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for GTP
Background Pseudomonas aeruginosa is an important opportunistic pathogen causing serious pulmonary, urogenital and systemic infections. P. aeruginosa injects four effector proteins into host cells via the type III secretion system. ExoY is one of these proteins. ExoY was originally classified as adenylyl cyclase with homology to the typical calmodulin-stimulated adenylyl cyclase exotoxins CyaA ...
متن کاملHeparin binding protein is increased in chronic leg ulcer fluid and released from granulocytes by secreted products of Pseudomonas aeruginosa.
Recently it was demonstrated by Gautam, et al. that release of neutrophil-borne heparin-binding protein (HBP), also known as CAP37/azurocidin, induced endothelial hyperpermeability and neutrophil efflux. Here, we show that chronic leg ulcer fluid, in contrast to wound fluid from acute wounds, contains highly increased levels of HBP. Immunohistochemistry demonstrated the presence of HBP in chron...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2000
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.182.5.1257-1263.2000